Development

MCIN scientists study many aspects of development in tissues ranging from peripheral to central targets. Questions addressed by researchers include those of structure, function and mechanisms for both neuronal (Bamburg, Rash, Tobet) and muscle cells (Garrity, Mykles) as well as cognitive processes in human subjects (Davies). Other model systems include crustaceans (Mykles), zebrafish (Garrity), rodents (Bamburg, Rash and Tobet). Studies examine processes including cell migration (Tobet), neurite extension and growth cone guidance (Bamburg), gap junction formation (Rash), limb regeneration (Mykles), heart formation (Garrity), and electroencephalographic changes during childhood (Davies). Recent articles in the area of developmental neurobiology from different faculty laboratories and links to the faculty home pages are given below.

 

Bamburg: We study the mechanisms of neuritogenesis, axonogensis and growth cone guidance and turning by extracellular guidance cues, including transmembrane signaling pathways and mechanisms that lead to actin reorganization during these processes.

Garvalov BK, Flynn KC, Neukirchen D, Meyn L, Teusch N, Wu X, Brakebusch C, Bamburg JR, Bradke F. Cdc42 regulates cofilin during the establishment of neuronal polarity. J Neurosci 27, 13117-13129 (2007). PMID:18045906  

Pak CW, Flynn KC, Bamburg JR. Actin binding proteins take the reigns in growth cones. Nature Rev Neurosci 9, 136-147 (2008). PMID:18209731  

Flynn KC, Pak CW, Shaw AE, Bradke F, Bamburg JR. Growth cone-like waves transport actin and promote axonogenesis and neurite branching. Develop Neurobiol 69, 761-779 (2009)  PMID:19513994

 

Davies: We study the development of cognitive and sensory processing in the human brain using electroencephalography (EEG) and event-related potentials (ERPs). We study both normal development and development in individuals with disabilities such as sensory processing disorders, autism, and ADHD.   Children and adolescents, ages 5 to 18, are participants in our studies, along with young adults.

Davies PL, Chang W-P, Gavin WJ.  Maturation of Sensory Gating Performance in Children with and without Sensory Processing Disorders.  Int J Psychophysiol 72,187-197 (2009). PMID: 19146890

Gavin WJ,  Davies PL.  Obtaining reliable psychophysiological data with child participants: Methodological considerations.  In L.A. Schmidt, S.J. Segalowitz (Eds.), Developmental Psychophysiology: Theory, systems, and methods (pp.424-447).  New York: Cambridge University Press (2007).

 

Garrity: We study the development of the heart using a zebrafish model system.

Rothschild SC, Easley CA 4th, Francescatto L, Lister JA, Garrity DM, Tombes RM. Tbx5-mediated expression of Ca(2+)/calmodulin-dependent protein kinase II is necessary for zebrafish cardiac and pectoral fin morphogenesis. Dev Biol 330, 175-84 (2009) PMID: 19345202

Ebert AM, McAnelly CA, Srinivasan A, Linker JL, Horne WA, Garrity DM. Ca2+ channel-independent requirement for MAGUK family CACNB4 genes in initiation of zebrafish epiboly. Proc Natl Acad Sci USA 105, 198-203 (2008) PMID: 18172207

 

Mykles: We study the regulation of growth and limb regeneration by steroid molting hormones (ecdysteroids) in crustaceans.

Kim HW, Chang ES, Mykles DL. Three calpains and ecdysone receptor in the land crab, Gecarcinus lateralis: Sequences, expression, and effects of elevated ecdysteroid induced by eyestalk ablation. J Exp Biol 208, 3177-3197 (2005). PMID: 16081615  

Kim HW, Lee SG, Mykles DL. Ecdysteroid-responsive genes, RXR and E75, in the tropical land crab, Gecarcinus lateralis: differential tissue expression of multiple RXR isoforms generated at three alternative splicing sites in the hinge and ligand-binding domains. Molec Cell Endocrinol 242, 80-95 (2005). PMID: 16150535

 

Rash: Using confocal immunofluorescence microscopy and freeze-fracture replica immunogold labeling (FRIL) electron microscopy, we have investigated the time of appearance and the composition of neuronal and glial gap junctions during early postnatal development in mouse and rat CNS.

Rash JE, Olson CO, Davidson KGV, Yasumura T, Kamasawa N, Nagy JI. Identification of connexin36 in gap junctions between neurons in rodent locus coeruleus. Neuroscience147, 938-956 (2007) PMID: 17601673  

Rash JE, Olson CO, Pouliot WA, Davidson KGV, Yasumura T, Furman CS, Royer S, Kamasawa N, Nagy JI, Dudek FE. Connexin36 vs. connexin32, “miniature” neuronal gap junctions, and limited electrotonic coupling in rodent suprachiasmatic nucleus (SCN). Neuroscience 149, 350-371 (2007) PMID: 17904757

 

Tobet: We study the role of steroid hormones and several neuronal signaling molecules that we refer to as ‘effectors’ on cell positions (e.g., cell migration) using immunocytochemical and in situ hybridization technique in vivo and organotypic hypothalamic slices and video microscopy in vitro.·       Bless EP, Walker HJ, Yu KW, Knoll JG, Moenter SM, Schwarting GA, Tobet SA. Live view of gonadotropin-releasing hormone containing neuron migration. Endocrinology146:, 463-468 (2005).  

Edelmann M, Wolfe C, Scordalakes EM, Rissman EF, Tobet S. Neuronal nitric oxide synthase and calbindin delineate sex differences in the developing hypothalamus and preoptic area. Develop Neurobiol 67, 1371-1381 (2007). PMID: 17638388   ·      

Tobet S, Knoll JG, Hartshorn C, Aurand E, Stratton M, Kumar P, Searcy B, McClellan K. Brain sex differences and hormone influences: A moving experience? J Neuroendocrinol21, 387-392 (2009). PMID: 19207813